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Postbac Seminar Series: June 20, 2017

Series: Science Skills; Speaking

Jun 20, 2017

This event is recommended for: Postbacs.

Science isn't complete until the results have been shared with others, and talking about your results is one of the important ways of making them public. The Postbac Seminar Series provides a unique opportunity for two Postbacs each month to present their research to a diverse audience of their peers.  The atmosphere is relatively informal and non-threatening.  The series allows Postbacs who attend to learn about the different types of biomedical research being conducted at the NIH while meeting other postbacs.  Read more about the seminar series.

This month's presenters are:

Maryknoll Palisoc, NCI

Title: Detection of Human and Viral IL-6 in Multicentric Castleman Disease

Summary: Multicentric Castleman Disease (MCD) is a heterogeneous group of lymphoproliferative disorders with a constellation of clinicopathological manifestations. Dysregulated cytokine activities have been implicated in the pathogenesis of MCD. A subset of MCD cases occurs in HIV-positive patients who are generally co-infected with Kaposi sarcoma-associated herpes virus/human herpes virus 8 (KSHV/HHV8). KSHV/HHV8 encodes a viral IL-6 (vIL-6), which can activate signaling pathways through a ubiquitously expressed receptor. Both hIL-6 and vIL-6 have been demonstrated to be crucial in the pathogenesis of MCD. However, the source of hIL-6 has not been fully demonstrated in MCD. We used a highly sensitive RNA in situ hybridization technique (RNAscope, ACDBio) to visualize cells that express hIL-6 transcripts in MCD while immunohistochemistry was used to determine the source of vIL-6. Our preliminary results suggest that different types of cells contribute to the cytokine dysregulation in MCD by expressing human and viral IL-6.

Bio: Maryknoll Palisoc graduated from Ohio State University in 2015 with a B.S. in Biochemistry and B.S. in Molecular Genetics and completed an M.S. in Biotechnology from Johns Hopkins University in May 2016. Joining NIH in June 2015, she has participated in different collaborative projects under the guidance of Dr. Stefania Pittaluga of the Hematopathology Section at NCI.

 

Maxim Jestin, NHGRI

Title: Metabolic decompensation: Role of TNFa in worsening liver mitochondrial disease during viral infection.

Summary: Mitochondrial hepatopathy is present in up to 20% of patients with mitochondrial disease. In addition to triggering mitochondrial hepatopathy, infection may also lead to potentially fatal episodes of metabolic decompensation characterized by lactic acidosis, hypoglycemia, and liver dysfunction/failure. Patients typically end up in the ICU, and if they survive, often suffer from long-standing complications. The mechanism underlying this disruption of metabolic equilibrium in mitochondrial hepatopathy during infection is not clear. To model metabolic decompensation during infection, we infected a mouse model of mitochondrial hepatopathy with mouse-adapted influenza, PR8 (H1N1). In profiling the systemic immune response due to influenza infection, TNFa appeared recurrently as a culprit in exacerbating metabolic dysfunction in our mice model. We show how blocking TNFa shows improvement, suggesting that targeting the immune system may be viable strategy for alleviating complications with metabolic decompensation.

Bio: McGuire Lab, NHGRI; University of Nevada, Reno

 

Assanatou Bamogo, NIAID

Title: Efficacy of Combined Maternal Immunization with DsCav1

Background: Based on the success of vaccination programs to prevent maternal and neonatal tetanus, maternal immunization (MI) has been well received in the United States and globally as a promising strategy for the prevention of other vaccine preventable diseases that threaten pregnant women and infants, such as influenza and pertussis. Currently, five infectious diseases are considered for MI, three with vaccines already available: influenza, pertussis and tetanus, and two for which vaccines specifically designed for MI are currently in clinical development: respiratory syncytial virus (RSV) and group B Streptococcus (GBS). We aim to study the interference of combined influenza, DTaP (diphtheria, tetanus, acellular pertussis) and DsCav1 vaccines through mice immunization.

Bio: Assanatou Bamogo graduated from the University of Central Florida in Orlando in 2016 with a B.S. in Health Science. As one of the NIH Undergraduate Scholarship Program scholar, she is working as a postbac in Dr Barney S. Graham's laboratory (Viral Pathogenesis laboratory of NIAID) focusing on RSV immunology and vaccine development.