Staff Scientist

The National Human Genome Research Institute (NHGRI), a major research component of the National Institutes of Health (NIH), and the Department of Health and Human Services (DHHS), is seeking exceptional candidates for a Staff Scientist position in the Intramural Research Program (IRP), Office of Core Facilities (OCF), Embryonic Stem Cell and Transgenic Mouse Core (ESCTMC). ESCTMC provides a shared service to NHGRI investigators and specializes in generating genetically engineered mice (GEM) via conventional transgenesis, gene editing using site-specific nucleases (CRISPR/Cas9 with in vitro and in vivo editing) and embryonic stem (ES) cell targeting. The core also provides cryopreservation services and assisted reproduction using in vitro fertilization, and intracytoplasmic sperm injection.

The Staff Scientist’s primary responsibilities will include the following: generation of gene edited mice via microinjection, electroporation, and in vivo delivery of CRISPR reagents to mouse embryos. Culture, maintenance, and transfection of mouse embryonic stem cells with subsequent microinjection of modified ES cells to mouse blastocysts would be advantageous. Specific surgical techniques would consist of embryo oviduct transfer, vasectomization, and artificial insemination. Mouse embryo manipulations would include in utero injections, cesarean section, and embryo dissection. Additionally, this position would require general knowledge of molecular biology with emphasis on genotyping via PCR and qPCR of mouse tissue and breeding colonies. Responsibilities also involve colony management, technology transfer and development. It will be necessary for the Staff Scientist to maintain databases, perform literature research and present results and analysis at meetings. The position requires additional support to core cryopreservation personnel for freezing of important mutant mouse strains and recovery via in vitro fertilization, and embryo transfer. Lastly, the Staff Scientist will provide technical support in project design for NHGRI investigators.

Candidates must have a M.D. or equivalent in Veterinary Medicine and hold a Laboratory Animal Technologist Certificate and must have a minimum of fifteen years’ experience in veterinary medicine and greater than 5 years mouse colony management with emphasis on generation ot transgenesis and gene editing. Additionally, it would be advantageous if candidates have a background in genetics and molecular biology.

The NIH is dedicated to building a community in its training and employment programs and encourages the application and nomination of qualified women, minorities, and individuals with disabilities.