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Associate Scientist / Scientist, Protein Purification

Adicet Bio, Menlo Park, California

Position Description:

JOIN US!
Adicet Bio, Inc is a clinical stage biotechnology company discovering and developing allogeneic gamma delta T cell therapies for cancer. We are advancing a pipeline of “off-the-shelf” gamma delta T cells, engineered with chimeric antigen receptors (CAR) and T cell receptor-like antibodies (TCRL), to enhance selective tumor targeting, facilitate innate and adaptive anti-tumor immune response, and improve persistence for durable activity in patients. Our approach to activate, engineer and manufacture allogeneic gamma delta T cell product candidates derived from the peripheral blood cells of unrelated donors allows us to generate new product candidates in a rapid and cost-efficient manner. For more information, please visit our website at http://www.adicetbio.com.
 
ASSOCIATE SCIENTIST/SCIENTIST, PROTEIN PURIFICATION
 
Adicet Bio is seeking a highly motivated team member who will be a key contributor in the protein purification laboratory, a person who possesses broad technical expertise and a desire to work at the bench. They will possess a working knowledge of protein and antibody purification and an ability to characterize purified materials using various biochemical and biophysical methodologies. The ideal candidate in addition would be in a unique position to contribute to the design of bispecific and antibody-like molecules and empirically study the impact of design on biological function.
 
Key Responsibilities:
  • Express recombinant proteins in mammalian cells (CHO, HEK) using transient transfection.
  • Purify recombinant proteins expressed in bacteria and mammalian cells.
  • Conduct parallel processing of small-scale benchtop purifications using gravity columns.
  • Use bead capture for high throughput small-scale immunoglobulin purification.
  • Author and execute methods on AKTA FPLC for automated routine purifications.
  • Complete routine sample analysis using HPLC system, SDS-PAGE and IEF.
  • Characterize antibody/antigen binding using ELISA and ForteBio Octet methods. Exposure to Luminex is a plus.
  • Maintain electronic notebook entries and protein inventory databases.
  • Present results at group and company meetings.

Qualifications:

  • BS or MS in biological sciences or a related field.
  • 5+ years of relevant academic and industry experience.
  • Broad technical experience with various antibody and protein separation techniques on small and large scale: affinity, ion exchange, hydrophobic, and size exclusion chromatography.
  • Hands-on experience with AKTA FPLC systems such as AKTA Prime, AKTA Explorer, AKTA Pure or similar.
  • Broad experience with analytical protein characterization methods, including HPLC (SEC, IEX), SDS-PAGE, IEF, Western.
  • Experience with antibody and protein labeling techniques: biotinylation, derivatization with fluorescent dyes (Alexa Fluor, DyLight, PE etc.)
  • Experienced in the use of expression platforms, both bacterial and mammalian (e.g., Expi293, ExpiCHO).
  • Antibody/antigen binding characterization using Octet® platform is a plus.
  • Ability to produce high quality and timely reports.
  • Strong interpersonal skills, ability to be productive in team environment, open minded to the diverse opinions of others.
  • Ability to multitask and meet deadlines in a fast-paced environment.
  • Openness to flexible working hours is preferred.
  • Highly motivated, organized, and disciplined record keeping.
  • Excellent verbal and written communication skills.

Adicet Bio requires all workers to be fully vaccinated against COVID-19, including the booster dose.

Benefits:
Adicet Bio offers both comprehensive benefits and Paid Time (PTO) plans for employees, which begin on the first day of employment, as well as the opportunity to work in a growing, dynamic environment.
 

To Apply:

Please visit this webpage and search for "Associate Scientist / Scientist, Protein Purification" to apply for the position: https://www.adicetbio.com/careers/openings/

Adicet Bio is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, gender identity or expression, sexual orientation, national origin, genetics, disability, age, or veteran status.