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Post Doctoral Associate

Yale School of Medicine, New Haven, Connecticut

Position Description:

1. In vivo animal and intestinal cell studies to investigate the role of serum glucocorticoid kinase 1 (SGK1) in regulating CFTR in the intestine- these studies involve the use of villin cre -SGK1 KO mice, and Nedd4-2 KO mice. Techniques used: Immunofluorescence label, confocal microscopy, qPCR, genotyping, animal husbandry, mouse in vivo intestinal studies, biochemistry, surface biotinylation, mass spec, phosphorylation assays, co-IP, electrophysiology Ussing chamber assays2. CFTR High Expresser Cells (CHE): Dr Ameen identified a unique subpopulation of enterocytes in rat and human small intestine that express very high levels of CFTR. Current studies are aimed at elucidating the fate specification and function of CHEs in health, CF and diarrheal diseases. Studies involve scRNA seq, organoid culture, fluorescent imaging, CRISPR-Cas, knock down of genes in organoids, human organoid culture, cell culture, rat intestinal in vivo physiology, cell pH studies.

Qualifications:

Cell Biology, physiology, molecular- cloning, tranfections, epithelial culture

To Apply:

Contact the PI: Nadia Ameen, Professor Pediatrics, Cellular and Molecular Physiology at Nadia.Ameen@yale.edu