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Glycobiology

National Institute of Dental and Craniofacial Research, Bethesda, MD and surrounding area

Position Description:

A postdoctoral fellowship is available for an outstanding scientist  to to characterize the O-glycome, i.e. all known sites mucin-type O-glycosylation in an organism. The O-glycome is defined by the substrate specificities of the multi-gene family of enzymes that catalyze the formation the core Ser/Thr-GalNAc linkage upon which O-glycans are built. Prior work from our group (https://irp.nih.gov/pi/lawrence-tabak - for example,  Fritz et al., Proc. Natl Acad. Sci. USA, 2004;101:15307; and, Raman et al., J. Biol. Chem. 2008;283:22942) together with more recent collaborative studies with the Drs. Kelly Ten Hagen and Nadine Samara (e.g. Ji et al., Nat Commun, 2018;9:3508) have defined the structural mechanisms that underlie the substrate specificities of these enzymes.

While approaches to delineating sites of O-glycosylation have been developed for simple, genetically engineered cell lines, to date there is no universal approach to study the entire O-glycome of a tissue extract or biological fluid. Therefore, our next goal is to develop appropriate chemical and/or enzymatic conditions that cleave O-glycans while leaving the modified core protein substrate assayable by mass spectrometry. In collaboration with the Ten Hagen lab (https://irp.nih.gov/pi/kelly-ten-hagen),  this platform will be used to deduce substrates contributing to biologically relevant phenotypes when specific GalNAc-Ts are genetically ablated in mice (e.g. Tian et al., PLoS One, 2015;10:e0115861) and flies (e.g. Zhang et al., Proc Natl Acad Sci U S A. 2014;111;7296).

Qualifications:

We are seeking a highly motivated candidate with a Ph.D. in the biomedical sciences, strong verbal and writing skills, and experience in protein chemistry, and/or glycobiology. Salary is commensurate with research experience and accomplishments.

To Apply:

Please submit your CV, bibliography, a brief statement of research interests, and the names of 3 references to:  Lawrence A. Tabak, DDS, PhD – Lawrence.Tabak@nih.gov

NIH/NIDCR is dedicated to building an inclusive and diverse community in its training and employment programs. Individuals from underrepresented ​racial and ethnic groups, individuals with disabilities, and women are always encouraged to apply for NIH/NIDCR employment. DHHS and NIH are equal opportunity employers.