Staff Scientist 1

The Staff Scientist will:

• Lead experimental studies of mitochondrial function and bioenergetics in human tissues and cellular models;
• Develop and optimize assays of oxidative phosphorylation, respiratory chain activity and organization, and mitochondrial cellular phenotypes;
• Analyze and interpret experimental data;
• Maintain rigorous standards for assay quality and reproducibility;
• Oversee the organization, inventory, storage, tracking, and stewardship of protocol-derived biospecimens in accordance with applicable regulatory, institutional, and research protocol requirements.

The Staff Scientist will also mentor junior staff and collaborate with NHGRI and other investigators on studies of mitochondrial dysfunction in human disease.

Salary will be commensurate with education and experience.

Candidates must have a Ph.D. and a minimum of four years of postdoctoral or equivalent research experience in mitochondrial biology, bioenergetics, or a related field.

Candidates should have demonstrated expertise in the design and execution of mitochondrial functional studies using human tissue samples, including extracellular flux analysis, high-resolution respirometry using Oroboros platforms, in-gel respiratory chain complex activity assays, blue native PAGE (BN-PAGE), analysis of respiratory chain super complex formation, and flow cytometry.

Experience with mammalian cell culture, including the generation, maintenance, and experimental use of mitochondrial DNA-depleted rho-zero (ρ0) cells, is also desirable.

Additional experience working with primary human tissues, isolated mitochondria, permeabilized cells, and complementary biochemical or molecular assays is highly desirable.

U.S. citizens and permanent residents are eligible to apply. NIH welcomes foreign nationals with the exception of individuals from this list.