Postdoctoral Fellow

The prospective candidate will develop a platform for CRISPR screening in 3-dimensional spheroids and organoids of disease models. Applicants with a background in cell/tumor biology, 3-dimensional models and high content imaging/analysis will receive preferential consideration. The selected candidate will closely work with a staff scientist as part of a multidisciplinary team with expertise in pooled and arrayed CRISPR and siRNA screens, quantitative cell imaging, assay development and drug screening.

The selected candidate will also build and characterize a platform using pooled and arrayed CRISPR libraries to interrogate genetic determinants of disease in 3-dimensional models. Candidates with experience in spheroid and organoid establishment, high content imaging and sequencing analysis are strongly encouraged to apply. Our laboratories are best known for their expertise in assay development, high-throughput screening and the application of innovative technologies.

Applying this expertise to a biologically relevant 3-dimensional model platform compatible with functional genomics will help bridge the gap between high throughput 2-dimensional preclinical testing and low throughput, expensive in vivo testing. The scope of this translationally focused resource is unparalleled outside of large pharmaceutical companies.

Prospective candidate will use cutting-edge technologies such as pooled and arrayed CRISPR screening, high content imaging, large particle sorting and Next-Generation Sequencing. They will establish and compare workflows of arrayed and pooled CRISPR screens in normal and disease models of 3-dimensional spheroids, then in organoids. Keeping accurate and complete records of all scientific experiments according to established procedures and ensuring that these records and raw data are properly retained is part of the position. Drafting protocols, reports, and abstracts, writing manuscripts, and preparing presentations, specifically to present findings at NCATS and to the scientific community, will also be part of the position.

Prospective candidates should be an early-career postdoctoral researchers (within 2 years of PhD completion) who are detail-oriented, team players with high scientific standards and the ability to work in an interactive environment. Being self-motivated and willing to learn new technologies and procedures is also required. A familiarity with mammalian cell culture, cloning, and the concepts of pooled and arrayed CRISPR screens, as well as sequencing platforms, is highly desirable.

U.S. citizens and permanent residents are eligible to apply. NIH welcomes foreign nationals with the exception of individuals from this list.